ABSTRACT
<p><b>AIM</b>To investigate the protective role of HO/CO systems in IL-1beta induced islest apoptosis and to explore the mechanisms of the protective effect of fructose-1, 6-disphosphate (FDP).</p><p><b>METHODS</b>The pancreases of the rats were removed to collect islets cells. The cells were incubated with IL-1beta with/or FDP. Cell activity, insulin secretion, HO-1 activity, CO content and apoptotic percentage were detected.</p><p><b>RESULTS</b>HO-1 activity and CO content of the normal control group were low. IL-1beta induced a significant decrease of cell activity and insulin release, flow cytometry analysis showed that apoptotic percentage of islet cells remarkably increased following the addition of IL-1beta, FDP obviously improved the islets cellular activity damaged by IL-1beta, and basic amount of insulin secretion and stimulated by high glucose were improved (P < 0.01). Content of CO and activity of HO-1 were higher in the IL-1beta group than the normal control group (P < 0.05), and there were significant differences between the FDP groups and IL-1beta group. FDP decreased cell apoptotic percentage. Activities of HO-1 and content of CO were higher than that in the IL-1beta group (P < 0.01).</p><p><b>CONCLUSION</b>FDP can attenuate the IL-1beta induced apoptosis of cultured beta cells, the mechanism of which may be improved HO-1 activity and CO content.</p>
Subject(s)
Animals , Female , Male , Rats , Animals, Newborn , Apoptosis , Carbon Monoxide , Metabolism , Cells, Cultured , Fructosediphosphates , Pharmacology , Heme Oxygenase (Decyclizing) , Metabolism , Physiology , Insulin , Bodily Secretions , Interleukin-1beta , Pharmacology , Islets of Langerhans , Cell Biology , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To study the vasodilation effect of the procyanidin (PC) extracted from grape seeds on rabbit thoracic aortic rings in vitro, decreasing blood pressure in vivo and the possible mechanism.</p><p><b>METHOD</b>Rabbits aortic rings were isolated and were divided into six groups including removal of endothelium, integrity of endothelium, 1 x 10(-5) mol X L(-1) indomethacin (Indo), 1 x 10(-5) mol x L(-1) propranolol (Prop), 1 x 10(-4) mol x L(-1) N(omega)-nitro-L-arginine (L-NNA) and 1 x 10(-5) mol x L(-1) methylene blue (MB). Then the thoracic aortic rings were treated with PC with cumulative concentrations of 1.25, 2.5, 5.0 mg x L(-1) respectively and the changes of tension were recorded, and investigate the effect of 40 mg x L(-1) PC on the contraction of aortic smooth muscles, thoracic aortic rings were pre-treated with NA (1 x 10(-8) to approximately 1 x 10(-5) mol x L(-1)), KCl (6.3 to approximately 100 mmol x L(-1)) and CaCl2 (1 x 10(-5) to approximately 1 x 10(-5) mol x L(-1)) followed by treatment with PC. Then, rabbits common carotid artery was intubated and arterial blood pressure in vivo was recorded. PC with cumulative concentrations of 4.0, 8.0, 16, 32, 64, 84 mg x kg(-1) was injected into vein and the changes of arterial blood pressure were observed.</p><p><b>RESULT</b>PC could relax isolated rabbit aorta and showedan obvious concentration-dependent relaxation (r = 0. 63, P < 0.001). The relaxant effect of PC was significantly reduced by removal of endothelium and by treatment with nitric oxide synthase inhibitor L-NNA, or guanylyl cyclase inhibitor MB. In addition PC could decrease the dose response curves of aortic rings to NA, KCl and CaCl2. PC has a significant concentration-dependent negative effect on arterial blood pressure in vivo (r = 0.92, P < 0.001).</p><p><b>CONCLUSION</b>PC has a vasodilation effect not only in an endothelium-dependent, nitric oxide involved manner, but in inhibition of calcium release and blockage of potential-dependent calcium channels. PC could decrease the rabbit's arterial blood pressure significantly in vivo.</p>
Subject(s)
Animals , Female , Male , Rabbits , Aorta , Physiology , Calcium Chloride , Pharmacology , In Vitro Techniques , Muscle Contraction , Muscle Relaxation , Muscle, Smooth, Vascular , Norepinephrine , Pharmacology , Potassium Chloride , Pharmacology , Proanthocyanidins , Pharmacology , Vasodilator Agents , PharmacologyABSTRACT
This article discusses the application of multimedia courseware to the physiology teaching.During making multimedia courseware hypertext interlinkage,diagram and simulating writing on blackboard should be applied.The physiology teaching should evoke study interest and activity of the students to improve teaching quality of physiology comprehensively.